Device
GFP-AID

Part:BBa_K812010:Design

Designed by: Cyrille Pauthenier   Group: iGEM12_Evry   (2012-09-18)


GFP fused with AID-tag (ubuquitinase E3 OsTirI recoginition domain)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1020
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1405


Design Notes

This part has been biobricked using PCR method and cloned in pSB1C3


Source

This part have been extracted by PCR from the plasmid pNHK60 obtained from the riken registry.

References